Publications and Preprints

Bold indicates authors from the Jiang Lab

*indicates equally contributing authors

#indicates corresponding authors

Ubiquitination Mechanisms

Xie G, Gao L, Lu R, Tian L, Zheng T, Li X, Dang Y, Cole PA, Yu X*, Jiang H*, Chen Z*. Biochemical Analysis of PD-L1 ubiquitination by CRL3SPOP, ARIH1, and NEDD4 family ubiquitin ligases. Structure. 2025, 33(8): 1304-1313. DOI: :10.1016/j.str.2025.05.005

Jiang H, Miller BD, Viennet T, Kim H, Lee K, Athanari H, Cole PA. Protein Semisynthesis Reveals Plasticity in HECT E3 Ubiquitin Ligase Mechanisms. Nature Chemistry, 2024, 16: 1894-1905. DOI: 10.1038/s41557-024-01576-z 

Jiang H, Chiang CY, Chen Z, Nathan S, D’Agostino G, Paulo JA, Song G, Zhu H, Gabelli SB, Cole PA. Enzymatic analysis of WWP2 E3 ubiquitin ligase using protein microarrays identifies autophagy-related substrates. Journal of Biological Chemistry, 2022, 298: 101854. DOI: 10.1016/j.jbc.2022.101854

Jiang H, Thomas SN, Chen Z, Chiang CY, Cole PA. Comparative analysis of the catalytic regulation of NEDD4-1 and WWP2 ubiquitin ligases. Journal of Biological Chemistry, 2019, 294:17421-36. DOI: 10.1074/jbc.RA119.009211

Chen Z, Jiang H, Xu W, Li X, Dempsey DR, Zhang X, Devreotes P, Wolberger C, Amzel LM, Gabelli SB#, Cole PA#. A tunable brake for HECT ubiquitin ligases. Molecular Cell, 2017, 66:345-57. DOI: 10.1016/j.molcel.2017.03.020

Jiang H, Dempsey DR, Cole PA. Ubiquitin ligase activities of WWP1 germline variants K740N and N745S. Biochemistry, 2021, 60:357-64. 10.1021/acs.biochem.0c00869

Wright KM, Jiang H, Xia W, Murphy MB, Boronina TN, Nwafor JN, Kim H, Iheanacho AM, Azurmendi PA, Cole RN, Cole PA. The C-Terminal of NaV1. 7 Is Ubiquitinated by NEDD4L. ACS Bio & Med Chem Au. 2023 Oct 13;3(6):516-27.

Wright KM*, Nathan S*, Jiang H, Xia W, Kim H, Chakouri N, Nwafor JN, Fossier L, Srinivasan L, Chen Z, Boronina T. NEDD4L intramolecular Interactions regulate its auto- and substrate NaV1.5 ubiquitination. Journal of Biological Chemistry. 2024 Feb 2:105715.

Mansur A, Joseph R, Kim ES, Jean-Beltran PM, Udeshi ND, Pearce C, Jiang H, Iwase R, Milev MP, Almousa HA, McNamara E. Dynamic regulation of inter-organelle communication by ubiquitylation controls skeletal muscle development and disease onset. Elife. 2023 Jul 11;12:e81966.

Salguero AL, Chen M, Balana AT, Chu N, Jiang H, Palanski BA, Bae H, Wright KM, Nathan S, Zhu H, Gabelli SB. Multifaceted Regulation of Akt by Diverse C-Terminal Post-translational Modifications. ACS Chemical Biology. 2022, 17, 1, 68-76.

Chemical Biology Tool Developments and Applications

Jang SS, Mandala S, Jiang H, Zhang X, Cole PA, Del Mármol J. A covalent recognition strategy enables conspecific mate identification. bioRxiv. 2025 Jun 3:2025-05.

Yeo MJ*, Zhang O*, Xie X*, Nam E*, Payne NC, Gosavi PM, Kwok HS, Iram I, Lee C, Li J, Chen NJ, Jiang H, Wang ZA, Lee K, Mao H, Harry SA, Barakat IA, Takahashi M, Waterbury AL, Barone M, Mattevi A, Carr SA, Udeshi ND, Bar-Peled L, Cole PA, Mazitschek R, Liau BB*, Zheng N*. Asymmetric Engagement of Dimeric CRL3KBTBD4 by the Molecular Glue UM171 Licenses Degradation of HDAC1/2 Complexes. Nature, 2024, 639, 232-240. DOI: 10.1038/s41586-024-08532-4

Xie X*, Zhang O*, Yeo MJ*, Lee C*, Harry SA, Paul L, Li Y, Payne NC, Nam E, Kwok HS, Jiang H, Mao H, Hadley JL, Lin H, Batts M, Gosavi PM, D’Angiolella V, Cole PA, Mazitschek R, Northcott PA, Zheng N*, Liau, BB*. KBTBD4 Cancer Hotspot Mutations Drive Neomorphic Degradation of HDAC1/2 Corepressor Complexes. Nature, 2024, 639, 241-249. DOI: 10.1038/s41586-024-08533-3

Lee K*, Barone M*, Waterbury AL, Jiang H, Nam E, DuBois-Coyne SE, Whedon SD, Wang ZA, Caroli J, Neal K, Ibeabuchi B, Dhoondia Z, Kuroda MI, Liau BB, Beck S#, Mattevi A#, Cole PA#. Uncoupling histone modification crosstalk by engineering lysine demethylase LSD1. Nature Chemical Biology, 2024. DOI: 10.1038/s41589-024-01671-9

Cao R, Gozlan O, Tveriakhina L, Zhou H, Jiang H, Cole PA, Aster JC, Sprinzak D, Blacklow SC. Structural Requirements for Activity of Mind bomb1 in Notch Signaling. Structure. 2024 Mar 3.

Iwase R, Dempsey DR, Whedon SD, Jiang H, Palanski BA, Deng B, Cole PA. Semisynthetic Approach to the Analysis of Tumor Suppressor PTEN Ubiquitination. Journal of the American Chemical Society, 2023, 145:6039-6044. DOI: 10.1021/jacs.2c13871

Wang ZA*, Markert JW*, Whedon SD*, Yapa Abeywardana M, Lee K, Jiang H, Suarez C, Lin H, Farnung L, Cole PA. Structural basis of Sirtuin 6-catalyzed nucleosome deacetylation. Journal of the American Chemical Society. 2023 Mar 17;145(12):6811-22.

Jiang H, Cole PA. N‐Terminal Protein Labeling with N‐Hydroxysuccinimide Esters and Microscale Thermophoresis Measurements of Protein‐Protein Interactions Using Labeled Protein. Current Protocols, 2021, 1:e14. DOI: 10.1002/cpz1.14

Jiang H, D'Agostino GD, Cole PA, Dempsey DR. Selective protein N-terminal labeling with N-hydroxysuccinimide esters. Methods in Enzymology, 2020, 639:333-53. DOI: 10.1016/bs.mie.2020.04.018

Dempsey DR, Jiang H, Kalin JH, Chen Z, Cole PA. Site-specific protein labeling with N-hydroxysuccinimide-esters and the analysis of ubiquitin ligase mechanisms. Journal of the American Chemical Society, 2018, 140:9374-8. DOI: 10.1021/jacs.8b05098

Natural Product Pharmacology

Zheng XY, Zhao X, Yang YF, Jiang HJ, Li W, Sun Y, Pu XP. Antioxidant, antiapoptotic and amino acid balance regulating activities of 1, 7-dihydroxy-3, 4, 8-trimethoxyxanthone against dimethylnitrosamine-induced liver fibrosis. Plos One. 2017 Dec 12;12(12):e0189344.

Li W, Jiang H, Ablat N, Wang C, Guo Y, Sun Y, Zhao X, Xu J, Zhang K, Ren R, Pu X. Evaluation of the acute and sub-chronic oral toxicity of the herbal formula Xiaoer Chaigui Tuire Oral Liquid. Journal of Ethnopharmacology. 2016 Aug 2;189:290-9.

Li W, Liu H, Jiang H, Wang C, Guo Y, Sun Y, Zhao X, Xiong X, Zhang X, Zhang K, Nie Z. (S)-Oxiracetam is the Active Ingredient in Oxiracetam that Alleviates the Cognitive Impairment Induced by Chronic Cerebral Hypoperfusion in Rats. Scientific reports. 2017 Aug;7(1):10052-.

Ren R, Shi C, Cao J, Sun Y, Zhao X, Guo Y, Wang C, Lei H, Jiang H, Ablat N, Xu J. Neuroprotective effects of a standardized flavonoid extract of safflower against neurotoxin-induced cellular and animal models of Parkinson’s disease. Scientific reports. 2016 Feb 24;6(1):1-3.